Check that all samples meet requirements.Bring the sample plate ON ICE to CPOS Genomics Core.Contact platform specialist for sample submission arrangement.Seal plate tightly with good adhesive film.Label CLEARLY on the side of the skirted 96-well PCR plate with your name, date and plate ID.Transfer 15 uL of DNA samples (50 ng/uL) to 96-well skirted plate according to sample layout spreadsheet.** Use 1 sample layout spreadsheet for each sample plate submitted.** Attach iScan sample layout spreadsheet after creating the service request. Completely fill in sample layout, sample ID and A260/280 ratio. if 8 samples/chip, use A1-H1 if 12 samples/per chip, use A1-D2 and continue in E2 and so on.
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**For custom Beadchip service, please contact Dr CHAN, Agnes (2831-5427) before sample submission. Submission of DNA samples for genotyping and methylation analysis Retain a softcopy of gel photo to be emailed to platform specialist.īelow is an acceptable example of gel photo: Please consult.įor both genotyping and methylation analysis, please examine DNA quality also on 0.8% agarose gel along with proper size marker (with good resolution). Normalized samples should be stored at -80 oC until sample submission.įor methylation analysis, sample quantity requirement depends on sample size. If these latter methods are used, it is safer to submit 20uL of DNA at 150 ng/uL for Qubit measurement. Alternative indirect methods such as UV spectrometry/NanoDrop can incorrectly report the concentration of dsDNA in your sample when single-stranded DNA, oligonucleotides, RNA, and/or proteins carried over from DNA extraction are present in the samples. Users are highly recommended to determine sample concentration by fluorometric assay, e.g. * Whole Genome Amplified DNA is not recommended. Dissolved in TE buffer(10 mM Tris, 1 mM EDTA), pH 8.0.Concentration MUST be normalized to 50 ng/uL**.Double-stranded, non-degraded high quality DNA*.Isolate DNA using high quality purification reagents/kits and dissolve/elute DNA in TE buffer (10 mM Tris, 1 mM EDTA), pH 8.0.ĭNA Sample Quality and Quantity requirements Please consult platform specialist before sample preparation. Preparation of DNA samples for genotyping and methylation analysis iScan Genotyping and Methylation Analysis.
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